More than 100 years have past since the battle between Jonathan Joestar and the vampire, Dio Brando. Now Dio's escape from the ocean depths awakens the psychic power known as "Stand" within 17-year-old Jotaro "JoJo" Kujo and his grandfather Josef Joestar. Now they must battle the immortal vampire Dio, who holds Jotaro's mother Holley prisoner in a nightmare-filled coma.

There is still no clue as how to defeat the Hanged Man, who reveals himself only through mirror reflections. How can they find a way to defeat this enigmatic Stand...?

While seeking solace in the nearest city, hordes of zombies descend upon Jotaro and his comrades, while "Justice" towers over them in the dark skies!

Seeking information on Dio's whereabouts, the companions encounter a gambler named D'Arby who claims to have the information they need. The catch is, they have to beat him in the ultimate wager - one where their very souls are at stake! Can the Joestar party outwit a master gambler at his own game?